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KMID : 0361019950380071025
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Korean Journal of Otolaryngology - Head and Neck Surgery
1995 Volume.38 No. 7 p.1025 ~ p.1031
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Long-term Culture of Human Nasal Microvascular Endothelial Cells and Capillary-like Tubulene Formation
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윤주헌
이정권/박인용/이제선/박남성
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Abstract
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Human nasal microvascular endothelial cells were isolated and cultured from the inferior turbinate. Endothelial cells grew rapidly to confluence and survived serial passages until the eighth subculture in a medium composed of MCDB 107, 10% fetal
bovine
serum, 75μg/ml endothelial cell growth supplement and 24units/ml insulin in the gelatin-coated 60mm dish. The cells were identified as endothelial cells by their morphology and immunofluoresence stain for human factor Ⅷ antigen. Capillary-like
tubule
formation was usually noted 4-6weeks after confluence. We succeeded in long-term culture of human nasal microvascular endothelial cells from the inferior turbinate. This will provide good model for studying the role of endothelial cells. (Korean
J
Otolaryngol 38:7, 1995)
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KEYWORD
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